pcr-rflp is a useful tool to distinguish between c. dubliniensis and c. albicans in cancer patients in iran
نویسندگان
چکیده
introduction: candida dubliniensis and c. albicans are very similar in morphology and phenotypic characteristics. approximation of this yeast species has caused major problems in identifying these two correctly. materials and methods: to distinguish among sixty yeast clinical isolates from patients with cancer, polymerase chain reaction - restriction fragment length polymorphism (pcr-rflp) was done. results: pcr-rflp of the its region showed different patterns between candida dubliniensis and c. albicans after digestion with enzymes blni. all of the clinical isolates were diagnosed as c .albicans. the results were confirmed by sequencing and rapd-pcr. conclusion: pcr-rflp would be a useful and applicable technique in clinical laboratories for discrimination of c. albicans and c. dubliniensis.
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عنوان ژورنال:
international journal of hematology-oncology and stem cell researchجلد ۴، شماره ۲، صفحات ۱۴-۱۸
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